G6046
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 | Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-G3BP (Cat. No. G6046). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. |
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G6046
Sigma
Anti-G3BP antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-G3BP1, Anti-GAP SH3 domain-binding protein, Anti-HDH-VIII, Anti-human DNA helicase VIII, Anti-Ras-GTPase-activating protein SH3-domain binding protein 1 |
Description
| Immunogen | synthetic peptide corresponding to amino acid residues 234-247 of human G3BP, conjugated to KLH via an added cysteine. The corresponding sequence differs by 4 amino acids in rat and mouse. |
| Preparation Note | In immunoblotting assays, it is highly recommended to dilute the antibody in 5% NFDM (w/v). |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~68 kDa |
| species reactivity | human, mouse (predicted), rat (predicted) |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 0.1-0.2 μg/mL using whole extract of human U-2-OS cells |
| indirect immunofluorescence: 2-5 μg/mL using human HeLa cells | |
| Gene Information | human ... G3BP1(10146) |
| rat ... G3bp1(171092) | |
| mouse ... G3bp1(27041) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Related Products
| Related product | G8046, Anti-phospho-G3BP (pSer149) antibody produced in rabbit |
